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cptms e01 mouse galectin 3 elisa kit aviscera bioscience  (ALPCO)


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    ALPCO cptms e01 mouse galectin 3 elisa kit aviscera bioscience
    Cptms E01 Mouse Galectin 3 Elisa Kit Aviscera Bioscience, supplied by ALPCO, used in various techniques. Bioz Stars score: 94/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cptms+e01+mouse+galectin+3+elisa+kit+aviscera+bioscience/Mouse+C-peptide+ELISA/ppr0784835-644-244-242
    Average 94 stars, based on 146 article reviews
    cptms e01 mouse galectin 3 elisa kit aviscera bioscience - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Isolation:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Purification:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    SYBR Green Assay:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Reverse Transcription:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Blocking Assay:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    CCK-8 Assay:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Plasmid Preparation:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Software:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.

    Real-time Polymerase Chain Reaction:

    Article Title: Galectin-3 Impairs Calcium Transients and β-Cell Function
    Article Snippet: 1214 58 Table S1 Key resources table 1215 Reagent or Resource Source Identifier Antibodies Rabbit anti-CD11c Cell Signaling Technology Cat# 97585 Guinea pig anti-insulin Abcam Cat# ab7842 Rat anti-insulin R&D Systems Cat# MAB1417 Rabbit anti-glucagon Cell Signaling Technology Cat# 2760 Mouse anti-galectin3 Abcam Cat# ab2785 Rabbit anti-CACNG1 Novus Cat# NBP2-76917 Rat anti-F4/80 Abcam Cat# ab6640 Mouse anti-flag Tag MBL Cat# M185 Rabbit anti-GFP Tag MBL Cat# 598 Rabbit anti-Phospho S473 Akt Cell Signaling Technology Cat# 4060 Rabbit anti-Akt Cell Signaling Technology Cat# 4691 Mouse anti-beta actin Proteintech Cat# 66009-l-lg Chemicals, Peptides, and Recombinant Proteins 60% high-fat diet Research Diets Cat# D12492 Collagenase, Type V Sigma-Aldrich Cat# C9263 Galectin-3 protein R&D Systems Cat# 1197-GA-050 Galectin-3-GFP protein This paper N/A L-Arginine Sigma-Aldrich Cat# A5131 GLP-1 (7-36) MCE Cat# HY-P0054 TRIzol RNA isolation reagent Thermo Fisher Scientific Cat# 15596026 RNA purification kit QIAGEN Cat# 74104 iTaq SYBR Green supermix Bio-Rad Laboratories Cat# 1725121 High-capacity cDNA reverse transcription kit Thermo Fisher Scientific Cat# 4368813 GB1107 MCE Cat# HY114409 TD139 Selleckchem Cat# S0471 Galectin-3 inhibitor Cpd47 Merck N/A Cacng1 blocking peptide Alomone labs Cat# BLP-CC111 Nimodipine Sigma-Aldrich Cat# N149 S961 Phoenix Pharmaceuticals Cat# 051-86 Clodronate Yeasen Biotechnology (Shanghai) Co., Ltd. Cat# 40337ES10 Critical Commercial Assays Mouse insulin ELISA kit ALPCO Cat# 80-INSMSU-E10 Rat insulin ELISA kit ALPCO Cat# 80-INSRTU-E10 Human insulin ELISA kit Mercodia Cat# 10-1132-01 Mouse glucagon ELISA EASYBIO Cat# 10-1281-01 59 kit Mouse somatostatin ELISA kit Phoenix Pharmaceuticals, Inc. Cat# EK-060-03 Mouse C-Peptide ELISA kit ALPCO Cat# 80-CPTMS-E01 Mouse Galectin-3 ELISA kit Aviscera Bioscience Cat# SK00199-03 ATP ELISA kit Promega Cat# FF2000 Glucose Uptake-GloTM Assay Promega Cat# J1343 Cell Counting Kit-8 DOJINDO Cat# CK17 GSH Kit Beyotime Cat# S0053 Experimental Models: Cell Lines MIN6 Gift from Dr. Xu in Institute of Biophysics, Chinese Academy of Sciences N/A INS1 ACCEGEN ABC-TC232S HEK293T ATCC N/A Human Pancreatic Islets Tianjin First Central Hospital N/A Mouse Pancreatic Islets This paper N/A Experimental Models: Organisms/Strains C57BL6/J Shanghai Research Center For Model Organisms Gal3 KO mice This paper db/db-Gal3 KO mice This paper db/db mice GemPharmatech Co,Ltd Recombinant DNA cacng1 c-myc plasmid Origene Cat# MR220452 cacng5 tv1 c-myc DDK mouse plasmid Origene Cat# MR228935 cacng5 tv2 c-myc DDK mouse plasmid Origene Cat# MR219914 Software and Algorithms ImageJ NIH https://imafej.nih.gov/ij/ Prism Graphpad https://www.graphpad.com Table S2 Primer sequences for mouse genes qPCR.



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